Polyblend Nanofibers to Regenerate Gingival Tissue: A Preliminary In Vitro Study

Aim: The regeneration of small periodontal defects has been considered an important divide and challenging issue for dental practitioners. aim this preliminary in vitro study was to analyze the effects polycaprolactone (PCL) nanofibers enriched with hyaluronic acid vitamin E vs. nude on gingival fibroblasts activity, innovative graft soft tissue purposes. Methods: Nanofibers were produced PCL (NF) or (NFE) by electrospinning technique. NF NFE stereologically morphologically characterized scanning electron microscope (SEM), composition analyzed infrared spectroscopy. Human obtained from one fragment (HGF) then seeded NF, NFE, plastic (CT). Cell adhesion morphology evaluated using SEM at 24 h cell viability after 24, 48, 72 alamarBlue® assay. Gene expression COL-I, LH2b, TIMP-1, PAX, VNC real-time RT-PCR samples run triplicate GAPDH used as housekeeping gene. Slot blot analysis performed immunoreactive bands revealed MMP-1 COL-I. YAP p-YAP Western membranes reprobed α-tubulin. Statistical performed. Results: IR spectrum presence NFE. At h, HGF adhered conserving fibroblast like morphology. seeding, statistically significant differences found proliferation cultured compared Expression genes (LH2b, MMP-1) proteins (COL-I) related collagen turnover a reduction COL-1 secretion cells CT; however, stimulated cross-linked deposition. Mechanosensor (PAX, VNC, YAP) upregulated while they decreased grown Conclusion: Preliminary data suggest that PCL-enriched could represent support induce proliferation, adhesion, cross-linking, reduce degradation, therefore favoring deposition connective tissue.